Journal: Nutrients

Article Title: Vitamin C Activates Osteoblastogenesis and Inhibits Osteoclastogenesis via Wnt/β-Catenin/ATF4 Signaling Pathways

doi: 10.3390/nu11030506

Figure Lengend Snippet: Expression of both osteoblast- and osteoclast-regulated proteins in vitamin C-treated rat tibias. ( A ) Western blot image of Wnt3a, β-catenin, and ATF4 and quantitative assay of Wnt3a, β-catenin, and ATF4 protein expression in vitamin C-treated rat tibias. ( B ) Western blot image of p-AKT, p-ERK, p-p38, and p-JNK and quantitative assay of p-AKT, p-ERK, p-p38, and p-JNK protein expression in vitamin C-treated rat tibias. Expression was quantified using ImageJ software relative to that of β-actin. Values represent the mean ± standard deviation. Values with different letters were significantly different according to Duncan’s multiple range test ( P < 0.05).

Article Snippet: Membranes were blocked with 5% bovine serum albumin prior to incubation with specific primary antibodies against BMP-2, RUNX2, Wnt3a, osteocalcin, COL-1 (Abcam, Cambridge, UK), SMAD1/5/8 (Santa Cruz Biotechnology, Dallas, TX, USA), ATF4 (Boster, Pleasanton, CA, USA), osteoprotegerin (OPG), RANK, RANKL (Bioss Antibodies, Woburn, MA, USA), TRAP, cathepsin K (GeneTex, Irvine, CA, USA), β-catenin, phosphorylated serine/threonine kinase (p-AKT), phosphorylated extracellular signal-regulated kinase (p-ERK), p-p38, phosphorylated c-Jun N-terminal kinase (p-JNK), and β-actin (Cell Signaling Technology, Danvers, MA, USA).

Techniques: Expressing, Western Blot, Software, Standard Deviation

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: E3 ubiquitin ligase BTRC inhibits the proliferation and tumor growth of glioma cells through the NFAT5/AQP4 axis

doi: 10.1007/s00432-025-06346-z

Figure Lengend Snippet: BTRC interacts with NFAT5 to influence its ubiquitination level. A Ubiquitination regulation relationship between NFAT5 and BTRC was performed by the UbiBrowser database. B Co-IP was used to verify the combination of BTRC and NFAT5. C The protein level of NFAT5 was detected using WB. D The protein level of NFAT5 was detected using WB. E The ubiquitination level of NFAT5 was detected by the IP experiment. n = 3. One-way or two-way ANOVA was used to analyze the differences among the groups, followed by Tukey's multiple comparisons test. ** indicates p < 0.01, *** indicates p < 0.001 compared with the oe-NC or oe-BTRC group

Article Snippet: To investigate the regulatory relationship between BTRC and NFAT5, cells were transfected with oe-BTRC or oe-NC and treated with a 30 μM protein synthesis inhibitor CHX (T1225, TargetMol, China).

Techniques: Ubiquitin Proteomics, Co-Immunoprecipitation Assay

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: E3 ubiquitin ligase BTRC inhibits the proliferation and tumor growth of glioma cells through the NFAT5/AQP4 axis

doi: 10.1007/s00432-025-06346-z

Figure Lengend Snippet: NFAT5 mediates the transcription of AQP4 affecting its expression in glioma Cells. A AnimalTFDB v4.0 was used to predict the binding sites of NFAT5 on the promoter region of AQP4, and the binding of NFAT5 and the AQP4 promoter region was detected by CHIP assay. B NFAT5 expression was detected by WB. C AQP4 expression was detected by RT-qPCR and WB. n = 3. A t-test or two-way ANOVA was used to analyze the differences between each group, followed by Tukey's multiple comparisons test. * indicates p < 0.05, *** indicates p < 0.001 compared with the IgG or si-NC group

Article Snippet: To investigate the regulatory relationship between BTRC and NFAT5, cells were transfected with oe-BTRC or oe-NC and treated with a 30 μM protein synthesis inhibitor CHX (T1225, TargetMol, China).

Techniques: Expressing, Binding Assay, Quantitative RT-PCR

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: E3 ubiquitin ligase BTRC inhibits the proliferation and tumor growth of glioma cells through the NFAT5/AQP4 axis

doi: 10.1007/s00432-025-06346-z

Figure Lengend Snippet: BTRC influences the malignant behavior of glioma cells through the NFAT5/AQP4 Axis. A The expressions of BTRC, NFAT5 and AQP4 were detected by WB. B Cell viability was detected by CCK8. C The apoptosis level was detected by flow cytometry. D Cell migration was detected by scratch assays. E Transwell was used to detect cell invasion. n = 3. A two-way ANOVA was used to analyze the differences among the groups, followed by a Tukey's multiple comparisons test. NS indicates p > 0.05, ** indicates p < 0.01, *** indicates p < 0.001 compared with the NC or oe-BTRC group

Article Snippet: To investigate the regulatory relationship between BTRC and NFAT5, cells were transfected with oe-BTRC or oe-NC and treated with a 30 μM protein synthesis inhibitor CHX (T1225, TargetMol, China).

Techniques: Flow Cytometry, Migration

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: E3 ubiquitin ligase BTRC inhibits the proliferation and tumor growth of glioma cells through the NFAT5/AQP4 axis

doi: 10.1007/s00432-025-06346-z

Figure Lengend Snippet: In vivo validation of BTRC's effect on glioma xenograft growth through the NFAT5/AQP4 axis. A Tumor images. B Tumor volume curve. C Tumor weight. D IHC was used to detect the positive rate of Ki67 in the tumor. E WB detection of BTRC, NFAT5, and AQP4 expression. n = 5. Differences among groups were analyzed using one-way or two-way ANOVA, followed by Tukey's multiple comparisons test. NS indicates p > 0.05, * indicates p < 0.05, *** indicates p < 0.001 compared with the NC or oe-BTRC group

Article Snippet: To investigate the regulatory relationship between BTRC and NFAT5, cells were transfected with oe-BTRC or oe-NC and treated with a 30 μM protein synthesis inhibitor CHX (T1225, TargetMol, China).

Techniques: In Vivo, Biomarker Discovery, Expressing

Journal: Oncology reports

Article Title: Gonadotropins promote human ovarian cancer cell migration and invasion via a cyclooxygenase 2-dependent pathway.

doi: 10.3892/or.2017.5784

Figure Lengend Snippet: Figure 1. FSHR and LHR were expressed in SKOV3 and HO8910 cells but did not affect cell proliferation and apoptosis. Expression of FSHR and LHR was examined by immunocytochemical staining (A), RT-PCR (B), and western blotting (C). HO8910 and SKOV3 cells were treated with FSH and LH (0, 100, and 500 mIU/ml) alone or in combination, but no changes in proliferation (D and E) and apoptosis (F) were observed.

Article Snippet: Antibodies specific to FSHR (cat. PB1120; 1:200) and LHR (cat. A01120; 1:200) were obtained from Boster.

Techniques: Expressing, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot